Table 1:

Examples of studies using s⁴U labeling to assess mRNA stability in cell lines of different organisms.

Human
Cell type	Concentration	Time of feed	Citation
BL41 (lymphoma)	100 µM s4U	1h	Dölken 2008, Friedel 2009
HeLa (cervical carcinoma)	100 µM s4U 250 µM s4U	1h 1h	Dölken 2008 Borowski 2014
Jurkat (T tymphocyte)	100 µM s4U 200 µM s4U	1h 1h	Dölken 2008 Blackinton 2016
DG75 (B lymphocyte)	100 µM s4U 500 µM s4U	1h 5–60 min	Dölken 2008 Windhanger 2012
HEK293T (embryonic kidney)	250 µM s4U 100 µM s4U 700 µM s4U	1h 20 min-22 d 1h	Borowski 2014 Duffy 2015 Gregersen 2014, Duffy 2015
293A (embryonic kidney	2.5 µM s4U	2h	Stubbs 2015
iPS (stem cell	400 µM s4U	2h	Russo 2017
LCL (lymphoblast)	200 µM s4U	2h	Duan 2013
MEWO (melanoma)	500 µM s4U	1h	Azarkh 2011
Primary T-cells	200 µM s4U	1-6h	Payne 2014
RAJI (lymphoblast)	300 µM s4U	30 min	Donato 2016
RCC (renal carcinoma)	2 µM s4U	2h	Bresson 2015
SH-SY5Y (neuroblastoma)	500 µM s4U	30 min	Schwarzl 2015
143B (osteosarcoma	250 µM s4U	1h	Borowski 2014
K562	500 µM s4U 100 µM s4U 1 mM s4U	5 min 4h 5 min	Schwalb 2016 Schofield 2018 Duffy 2018
MDA-MB-231 (breast cancer)	200 µM s4U	20 min	Cho 2018
MCF7 (breast cancer)	400 µM s4U	2h	Schwanhausser 2011
Mouse
NIH-3T3 (fibroblasts)	200 µM s4U 500 µM s4U 400 µM s4U	1h <30 min 2h	Dölken 2008 Dölken 2008 Schwanhausser 2011
RAW 264.7 (macrophages)	100 µM s4U	1h	Dölken 2008
SVEC 4–10 (endothelial)	100 µM s4U	1h	Dölken 2008
MEF	1 mM s4U 200 µM s4U	1h 2h	Schofield 2018 Kenzelmann 2007
Primary T-cells	250 µM s4U	15 min	Li 2017
Dendritic cells	150 µM s4U	45 min	Rabani 2011
Yeast
S. cerevisiae + UPRT	200 µM 4-thiouracil	3h	Muchel 2011
S. cerevisiae + hENT1	500 µM s4U	3-24 min	Miller 2011
S. cerevisiae S. pombe	5 mM 4-thiouracil 500 µM s4U	6 min 6 min	Sun 2012 Sun 2012
S. cerevisiae + FUI1	100 µM s4U	2-7 min	Swaitkowska 2012
S. cerevisiae	500 µM 4-thiouracil	3-100 min	Neymotin 2014
S. cerevisiae + FUI1	500 µM 4-thiouracil	1.5-5 min	Barrass 2015
S. cerevisiae	50 µM 4-thiouracil	16h	Miller 2018
Other
H. volcanii	300 µM 4-thiouracil	45 min	Knüppel 2017
S. acidocaldarius	135 µM 4-thiouracil	45 min	Knüppel 2017
D. melanogaster kc167	500 µM s4U	5-20 min	Pai 2017
D. melanogaster kc167	300 µM s4U	30-450 min	Chen and van Steensel 2017
D. melanogaster S2	200 µM s4U	2-6h	Khong 2017
D. rerio (zebrafish) embryo	1 nL 50 mM s4UTP	0-9 cell divisions	Heyn 2014