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. Author manuscript; available in PMC: 2019 Sep 30.
Published in final edited form as: ACS Biomater Sci Eng. 2018 Nov 4;4(12):4354–4366. doi: 10.1021/acsbiomaterials.8b01142

Figure 5.

Figure 5.

Effects of Wnt Inhibition on neural patterning of ECM treated-hiPSCs. The cells treated with ECMs and ±Wnt inhibitor IWP4 were analyzed for forebrain and hindbrain markers. (A) Fluorescent images of forebrain markers (PROX1, TBR1) and hindbrain markers (HOXB4, ISL1) at day 20 with ± IWP4. Scale bar: 100 μm. (B) Quantification of hindbrain (i) and forebrain (ii) marker expression at day 20 with ± IWP4 by flow cytometry (n = 3). Control indicates Geltrex conditions. * and ** indicate p < 0.05 (two-way ANOVA, see Supporting Information Table S3).