Skip to main content
. Author manuscript; available in PMC: 2019 Sep 30.
Published in final edited form as: ACS Biomater Sci Eng. 2018 Nov 4;4(12):4354–4366. doi: 10.1021/acsbiomaterials.8b01142

Figure 6.

Figure 6.

Effects of cytochalasin D treatment on neural patterning of ECM treated-hiPSCs. The cells treated with ECMs and ± cyto D at day 16 were analyzed for forebrain and hindbrain markers. (A) Representative flow cytometry histograms of forebrain marker (PROX1) and hindbrain marker (HOXB4) at day 20 under ± cyto D. Black line, negative control; red line, marker of interest. (B) Expression of (i) hindbrain marker HOXB4 and (ii) forebrain marker PROX1 at day 21 under ±cyto D quantified by flow cytometry (n = 3). Control indicates Geltrex conditions. * indicates p < 0.05 (two-way ANOVA, see Supporting Information Table S3).