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. 2019 Sep 30;14(9):e0223026. doi: 10.1371/journal.pone.0223026

Table 3. Effect of long-term N fertilization on soil properties.

pH TNa (g kg-1) NO3- N (mg kg-1) NH4+ N (mg kg-1) SOCa (g kg-1) C:N
Wheat season
Control 8.12 ± 0.017 ab 1.19 ± 0.013 c 5.86 ± 0.87 b 3.22 ± 0.64 a 20.57 ± 0.55 b 17.35 ± a 0.37 a
Low-N 8.00 ± 0.018 b 1.28 ± 0.019 b 11.07 ± 1.37 ab 2.81 ± 0.33 a 22.11 ± 0.18 a 17.32 ± a 0.16 a
High-N 7.91 ± 0.016 c 1.32 ± 0.015 a 19.86 ± 5.08 a 2.69 ± 0.06 a 21.97 ± 0.20 a 16.69 ± a 0.24 a
Source of variation
df 2 2 2 2 2 2
SS 0.08 0.03 400 0.60 5.70 1.48
F 26.22 33.49 6.01 0.33 12.97 2.12
P ≤ 0.001 ≤ 0.001 ≤ 0.05 0.68 ≤ 0.01 0.20
Maize season
Control 7.96 ± 0.028 a 1.15 ± 0.016 b 3.06 ± 0.37 b 1.54 ± 0.23 a 18.53 ± 0.45 b 16.43 ± a 0.18 a
Low-N 7.79 ± 0.028 b 1.24 ± 0.024 a 14.65 ± 1.13 a 1.79 ± 0.50 a 20.28 ± 0.57 a 16.38 ± a 0.26 a
High-N 7.80 ± 0.006 b 1.27 ±0.016 a 14.87 ± 1.06 a 1.29 ± 0.12 a 20.73 ±0.32 a 16.33 ± a 0.08 a
Source of variation
df 2 2 2 2 2 2
SS 0.07 0.03 364 0.49 10.75 0.02
F 29.78 9.28 40.87 0.65 8.69 0.07
P ≤ 0.001 ≤ 0.01 ≤ 0.001 0.44 ≤ 0.01 0.93

Data are the means ± standard error (n = 4).

a TN = total nitrogen, SOC = soil organic carbon.

b Different letters in a column indicate significant differences among treatments at P ≤ 0.05 as determined by fisher’s least significant difference (LSD). Control (No N added), Low (182 kg ha-1 of N), and High additions (225 kg ha-1 of N) in each crop season.