Fig 4. CRT loss initiates nonapoptotic cell-death response leading to the complete cell disintegration.

Morphological changes occurring in shCRT and shCont transduced melanoma cells M727 over the period of 72 hours. Bright field image sequence (t1–t6) was taken using 20× objective of the Olympus VivaView FL motorized inverted microscope integrated into TC chamber. CRT, calreticulin; shCont, short hairpin RNA targeting Control; shCRT, short hairpin RNA targeting Calreticulin; TC, tissue culture.