Skip to main content
. 2019 Sep 30;14(9):e0223252. doi: 10.1371/journal.pone.0223252

Fig 3. DKK1 promotes inflammatory cytokines secretion in hepatocellular carcinoma cell lines.

Fig 3

(a) DKK1 knockdown was confirmed by PCR and qPCR (S.2). (b) Relative mRNA expression of IL-6, IL-1ẞ, TNF-α, and TGF-β1 after PLC/PRF/5 and HepG2/C3A cells were exposed to DKK1 for 48 h and 72 h, respectively, and following DKK1 knockdown. Expression was measured with qPCR and normalized to that of GAPDH. (c, d, e) IL-6, TNF-α and TGF-β1 secretion levels in the cell supernatants were measured after DKK1 treatment and in the absence of DKK1 expression by sandwich ELISA. Data are expressed as the mean ± SD of triplicates. * p <0.05, ** p <0.01, *** p <0.001, as indicated.