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. 2019 Sep 30;14(9):e0223252. doi: 10.1371/journal.pone.0223252

Fig 5. TGFB1 knockdown suppresses the DKK1 proinvasive effect.

Fig 5

(a) TGF-β1 knockdown was confirmed by PCR and qPCR (S. 2). (b) Relative mRNA expression of MMP-2 and MMP-9 following DKK1 treatment in the presence or the absence of TGF-β1 expression (siTGFB1). Expression was measured with qPCR and normalized to that of GAPDH. (c) Western blot and densitometry analysis to calculate the normalized ratio of MMP-2 and MMP-9 to β-actin protein expression. Data are expressed as the mean ± SD from 3 different experiments. The siTGFB1 effects on DKK1 induced migration (d) and invasion (e) were assessed by the Transwell Boyden chamber assay. Representative photographs of migratory (d) and invasive (e) cells. All of the experiments were repeated 3 times. Data are expressed as the mean ± SD of triplicates. * p <0.05, ** p <0.01, *** p <0.001, as indicated.