Figure 2.

Workflow of human β-cell heterogeneity analysis. Single cell RNA sequencing data from pancreatic islet cells was used to perform single-cell analysis. Here, four different β-cell subpopulations were found with 488 enriched genes in total (enriched genes in all four subpopulations). Each dot represents a cell, with each color depicting the four distinct subpopulations. Other islet cells are shown in gray for reference. Pseudotime analysis was performed encompassing 6,241 β-cells and using the 488 enriched genes originated in the single-cell analysis. Here, the color of each cell emphasizes the distribution of cells from the four subpopulations observed in the single-cell analysis along the pseudotime trajectory [21], [47]. Adapted from Ref. Xin et al. [21].