Fig 8. Fluorescent intensity at 520 nm (RNase A/B) and 556 nm (DNase I) versus time for nucleases in 1 mM buffers lysed with Lyse-It at 50% power for 60 seconds (A) 20 pM RNase A (B) 46 pM RNase B, (C) 10.5 nM DNase I.

In general, HEPES buffer was seen to protect the nuclease from becoming more inactive as compared to Tris-EDTA or DI water.