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. 2019 Sep 30;14(9):e0223008. doi: 10.1371/journal.pone.0223008

Table 1. RNase B rates and percentage still active post conventional heating for 1 minute between 40°C and 80°C.

Conventional Heating -Temperature (°C) Rate (Fluorescent Intensity per Second) Nuclease Percentage Still Active
RNase B
Pre (RT) 136.7 ± 2.1 100%
40 136.7 ± 2.1 100%
50 143.6 ± 6.9 100%
60 130.9 ± 6.8 95.7%
70 120.6 ± 6.6 86.3%
80 103.0 ± 6.9 75.3%