Table 1. RNase B rates and percentage still active post conventional heating for 1 minute between 40°C and 80°C.
Conventional Heating -Temperature (°C) | Rate (Fluorescent Intensity per Second) | Nuclease Percentage Still Active |
---|---|---|
RNase B | ||
Pre (RT) | 136.7 ± 2.1 | 100% |
40 | 136.7 ± 2.1 | 100% |
50 | 143.6 ± 6.9 | 100% |
60 | 130.9 ± 6.8 | 95.7% |
70 | 120.6 ± 6.6 | 86.3% |
80 | 103.0 ± 6.9 | 75.3% |