KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
Antibodies | ||
| Mouse monoclonal anti-Acetylated-tubulin | Sigma | Cat#- T7451; RRID:AB_609894 |
| Rabbit polyclonal anti-Foxj1 | Sigma | Cat#- HPA005714; RRID:AB_1078902 |
| Mouse monoclonal anti-GLAST | Miltenyi Biotech | Cat#- 130-095-822; RRID:AB_10829302 |
| Mouse monoclonal anti-HA-tag | Genescript | Cat#- A01244-100; RRID:AB_1289306 |
| Chicken polyclonal anti-HA-tag | Aves | Cat#- ET-HA100; RRID:AB_2313511 |
| Rabbit polyclonal anti-EGFR | Genetex | Cat#- GTX100448; RRID:AB_1950176 |
| Rabbit polyclonal anti-pEGFR | Abcam | Cat#- ab5644; RRID:AB_305012 |
| Rabbit polyclonal anti-AKT | Cell Signaling | Cat#- 4691T; RRID:AB_915783 |
| Rabbit polyclonal anti-pAKT | Cell Signaling | Cat#- 4060T; RRID:AB_2315049 |
| Rabbit polyclonal anti-ERK | Cell Signaling | Cat#- 9102S; RRID:AB_330744 |
| Rabbit polyclonal anti-pERK | Cell Signaling | Cat#- 9101S; RRID:AB_331646 |
| Goat polyclonal anti-Numb | Abcam | Cat#- ab4147; RRID:AB_304320 |
| Rabbit polyclonal anti-Notch | Cell Signaling | Cat#- 4147S; RRID:AB_2153348 |
| Mouse monoclonal anti-S100b | Sigma | Cat#- SAB1402349; RRID:AB_10640343 |
| Chicken polyclonal anti-GFP chicken | Aves | Cat#- GFP1020; RRID:AB_10000240 |
| Chicken polyclonal anti-GFAP | Aves | Cat#- GFAP; RRID:AB_2313547 |
| Mouse monoclonal anti-GFAP | Sigma | Cat#- G3893; RRID:AB_477010 |
| Rabbit polyclonal anti-γ-Tubulin | Sigma | Cat#- T5192; RRID:AB_261690 |
|
Bacterial and Virus Strains | ||
| Lentivirus WT-Numb-GFP | This paper | N/A |
| Lentivirus S276S-Numb-GFP | This paper | N/A |
| Lentivirus WT-EGFR-HA | This paper | N/A |
| Lentivirus P67A-EGFR-HA | This paper | N/A |
|
Chemicals, Peptides, and Recombinant Proteins | ||
| Erlotinib | Selleckchem | S7786 |
| Mek Inhibitor | Selleckchem | S1036 |
| EGF | Invitrogen | PHG0311 |
|
Critical Commercial Assays | ||
| Q5 site directed mutagenesis kit | New England Biolabs | E0554S |
| Superscript Vilo cDNA synthesis kit | Invitrogen | 11754050 |
|
Deposited Data | ||
| Ependymal microarray data | This paper | GEO: GSE124518 |
|
Experimental Models: Cell Lines | ||
| 293T17 | ATCC | CRL-11268 |
| MDCK | ATCC | PTA-6500 |
|
Experimental Models: Organisms/Strains | ||
| FOXJ1-GFP | Ostrowski et al., 2003 | N/A |
| nestin-CreERtm4 | Kuo et al., 2006 | N/A |
| FOXJ1-Cre | Zhang et al., 2007 | N/A |
| numblikeKO/+ | Petersen et al., 2002 | N/A |
| CAG-EGFP | Kawamoto et al., 2000 | N/A |
| numbflox/+ | Zhong et al., 2000 | N/A |
| R26R-tdTomato | Arenkiel et al., 2011 | N/A |
|
Oligonucleotides | ||
| Refer to Table S1 | N/A | |
|
Recombinant DNA | ||
| PWPXLD-WT-Numb-GFP | This paper | N/A |
| PWPXLD-S276D-Numb-GFP | This paper | N/A |
| PWPXLD-WT-EGFR-HA | This paper | N/A |
| PWPXLD-P667-EGFR-HA | This paper | N/A |