Figure 7.
Effect of NTZ analogs on OM usher assembly. Structures of NTZ-derived analogs (A). Strain BW25113/pMJ3 (PapCHis) was grown in the presence of 32 μm NTZ or the indicated NTZ derivatives (B). OM fractions isolated from normalized bacterial cultures were subjected to SDS-PAGE and Coomassie Blue staining to observe PapC and the overall OMP profile (top). Samples were also probed with anti-His tag antibody to visualize PapC (bottom). PapC levels were measured by densitometry of the anti-His tag blot, and percentage PapC levels were calculated relative to 0 μg/ml NTZ (C). The graph shows individual data points and means ± S.D. (error bars) from four independent experiments. *, p < 0.05; ***, p < 0.001; ****, p < 0.0001 for comparison of each compound with DMSO vehicle control.