Figure 4.

Characterization of the triple-helix global conformation and stability. A, CD wavelength scan at 4 °C of each CMP (0.5 mg/ml) in 10 mm acetic acid from 190 to 260 nm. B, temperature dependence of the CD signal at 224 nm for each CMP (1 mg/ml) in 10 mm acetic acid from 0 to 70 °C. Fraction folded was based on the assumption that the peptides are fully folded at 0 °C and fully unfolded at 70 °C. The dashed line denotes a 50% folded population. deg, degrees.