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. 2019 Jul 23;294(39):14454–14466. doi: 10.1074/jbc.RA119.008231

Figure 4.

Figure 4.

ANKRD9 transition to rods is reversible with guanosine addition. a, left, IMPDH2 rod formation is reversed with guanosine addition. HeLa cells were treated for 1 h with 10 μm ribavirin to induce IMPDH2 rod formation and immunostained for IMPDH2 (green); right, HeLa cells were treated with 10 μm ribavirin for 1 h to induce rods formation, then 100 μm guanosine was added for 60 min and cells were immunostained for IMPDH2. n = 3 for each condition. Scale bar, 20 μm. b, control, FLAG-ANKRD9 forms rods following incubation of transfected HeLa cells in 0.1% FBS for 48 h. c, the same treatment, followed by incubation with 100 μm guanosine for 1 h. The ANKRD9 rods disappear and ANKRD9 shows a vesicle-like pattern. n = 3. Scale bar, 20 μm. d, percentage of cells with ANKRD9 vesicle-like structures or rods at 10 and 60 min after addition of guanosine. n = 3 for each time point. Error bar represents S.D. ****, p value < 0.0001., unpaired t test. Scale bar, 20 μm.