Figure 6.

A conserved CysCys motif in ANKRD9 is required for transition from vesicle-like structures to rods. a, structural model of ANKRD9 with conserved Cys residues highlighted in blue; the boxed area shows the position of Cys¹⁰⁹ and Cys¹¹⁰ in the loop. b, multiple sequence alignment of the Cys¹⁰⁹Cys¹¹⁰-containing region illustrates conservation of this motif. c, HeLa cells were transfected with WT ANKRD9 (control) or indicated ANKRD9 mutants and placed in 0. 1% FBS-containing medium for 48 h. Unlike WT, none of the mutants formed rods under these conditions. n = 3 for each condition. Scale bar, 20 μm. d, quantification of ANKRD9 mutant rod formation where vesicle-like structures are in black and rods are in gray. Unlike WT, none of the mutants formed rods under these conditions. n = 3. ****, p < 0.0001 unpaired t test. Error bar represents S.D.