Figure 2.

HCV does not alter global lysosomal proteolytic activity. A, hUH-7.5–GFP–IPS cells were treated with 25 nm bafilomycin A1 (BafA) overnight and subsequently exposed to Magic Red cathepsin B. Scale bars represent 20 μm. B, cell lysate cathepsin assay. Huh-7.5 cells were incubated as described for A, and fluorescence measurements were taken as described under “Experimental procedures.” *, p < 0.001 by the Mann–Whitney rank sum test. C, Huh-7.5 cells were treated with 25 nm bafilomycin A1 overnight, and a 3000 × g pellet was assessed by SDS-PAGE.