Figure 4.

JFH-1 infection alters the lysosomal Arf-like GTPase, Arl8b. A, Huh-7.5 cells were infected with JFH-1 and assessed for Arl8b expression by Western blotting. B, densitometry analysis of the changes in Arl8b levels, normalized to GAPDH levels, as determined from Western blotting (n = 3). C, quantification of Arl8b mRNA levels normalized to GAPDH. D, Huh-7.5–RFP–IPS cells were transfected with Arl8b–GFP and stained with phalloidin to define cell boundaries. E, overexpression of Arl8b–GFP in Huh-7.5–RFP–IPS cells. The cells were then immunostained for LAMP-2 to identify lysosomes. F, Huh-7.5 cells were transfected with Arl8b–GFP, treated with EBSS, and immunostained for LC3 to identify autophagosomes. Scale bars represent 10 μm. Cont, control.