Figure 7.

Preventing NOTCH2 signal activation reverses the effect of the Hajdu Cheney mutation on TNFα-induced osteoclastogenesis. BMMs derived from 2-month-old Notch2^tm1.1Ecan mice and control littermates were cultured with M-CSF at 30 ng/ml and TNFα at 200 ng/ml in the presence of control anti-ragweed at 10 or 20 μg/ml (Ctrl) or anti-NOTCH2 NRR (N2NRR) at 10 μg/ml (A), or anti-JAG1 (JAG1) at 20 μg/ml (B) for 6 days. A and B, representative images of TRAP-stained multinucleated cells obtained after 6 days of culture are shown. The scale bars in the right corners represent 500 μm. TRAP-positive cells with more than three nuclei were considered as osteoclasts, and values are means ± S.D. (top and bottom right); n = 4 biological replicates for control (white bars) and Notch2^tm1.1Ecan (black bars). *, significantly different between Notch2^tm1.1Ecan and control, p < 0.05. #, significantly different between anti-NOTCH2 NRR or anti-JAG1 and control anti-ragweed antibodies, p < 0.05.