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. 2019 Sep 23;8:e48284. doi: 10.7554/eLife.48284

Figure 3. Relative mRNA expression of Notch target genes in muscle stem cells in regenerating versus overloaded muscle.

(A) Immunostaining of laminin α2 (LNα2; red) and YFP (green) in isolated myofibers derived from Pax7CreERT2+::RosaYFP (Rosa-YFP) mice at 4 days after tenotomy. Nuclei were counterstained with DAPI. Scale bar: 10 µm. (B) Immunostaining of Pax7 (red), laminin α2 (LNα2; green), and EdU (white) in sections from C57BL/6 mice at 4 days after tenotomy. Nuclei were counterstained with DAPI. Arrow or arrowhead indicate EdU+ or EdU− Pax7+ cells. Scale bar: 20 µm. (C) RNA-seq analyses of Notch, myogenic, and cell-proliferation-related genes in MuSCs from sham or overloaded plantaris muscle of Rosa-YFP at 4 days after tenotomy. (D, E) Mononuclear myogenic cells were isolated from intact muscle (Cont; red), damaged muscle at 2 days after cardiotoxin injection (CTX 2d), sham-operated muscle (Cont; blue), or overloaded plantaris muscle of C57BL/6. Relative expression of Hey1 and Heyl (D) and three other canonical Notch target genes (Col5a1, Col5a3, and Col6a1) were compared between Cont and CTX/tenotomy samples (E).

Figure 3.

Figure 3—figure supplement 1. Expression of Notch, myogenic, and cell-proliferation-related genes in MuSCs from intact or damaged muscles.

Figure 3—figure supplement 1.

MuSCs were prepared from intact or damaged hindlimb muscles at 2 days after CTX injection. The heat map shows the gene-expression profile obtained using microarray data (accession number: GSE56903).