Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Hey1-KO | PMID: 15680351 | MGI:3574059 | Kokubo et al., 2005 |
| Strain, strain background (Mus musculus) | Heyl-KO | PMID: 21989910 | MGI:5302514 | Fukada et al., 2011 |
| Strain, strain background (Mus musculus) | Pax7CreERT2/+ | The Jackson Laboratory PMID: 19554048 | JAX:# 012476 RRID:IMSR_JAX:012476 |
|
| Strain, strain background (Mus musculus) | Rosa26EYFP | The Jackson Laboratory PMID: 19554048 | JAX:# 006148 RRID:IMSR_JAX:006148 |
|
| Antibody | anti-CD31-FITC (rat monoclonal) | BD Biosciences | Cat# 558738 RRID:AB_397097 |
1:400 |
| Antibody | anti-CD45-FITC (rat monoclonal) | eBioscience | Cat# 11-0451-82 RRID:AB_465050 |
1:800 |
| Antibody | anti-Sca1-PE (rat monoclonal) | BD Biosciences | Cat# 553336 RRID:AB_394792 |
1:400 |
| Antibody | anti-Satellite cells (rat monoclonal) | Fukada et al., 2004 ECR 296, 245–255. | 1:200 | |
| Antibody | anti-Dystrophin (rabbit polyclonal) | Abcam | Cat# ab15277, RRID:AB_301813 | 1:800 |
| Antibody | anti-eMyHC (mouse monoclonal) | DSHB | Cat# F1.652 RRID:AB_528358 |
1:2 (Supernatant) |
| Antibody | anti-GFP (YFP) (goat polyclonal) | SICGEN | Cat# AB0020-200, RRID:AB_2333099 | 1:1000 |
| Antibody | anti-GFP (YFP) (rabbit polyclonal) | MBL | Cat# 598S, RRID:AB_591816 | 1:1000 |
| Antibody | anti-Ki67 (rat monoclonal) | Thermo Fisher Scientific | Cat# 14-5698-82 RRID:AB_10854564 |
1:200 |
| Antibody | anti-Laminin a2 (rat monoclonal) | Enzo Life Sciences | Cat#ALX-804–190 C100, RRID:AB_2051764 | 1:200 |
| Antibody | anti-M-cadherin (rabbit polyclonal) | Ojima et al., 2004 | 1:1000 | |
| Antibody | anti-MyoD (rabbit polyclonal) | Santa Cruz | Cat# sc-760 RRID:AB_2148870 |
1:200 |
| Antibody | anti-MyoD (mouse monoclonal) | BD Biosciences | Cat# 554130 RRID:AB_395255 |
1:200 |
| Antibody | anti-Pax7 (mouse monoclonal) | DSHB | Cat# PAX7 RRID:AB_528428 |
1:2 (Supernatant) |
| Commercial assay or kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye | Thermo Fisher Scientific | #C10340 | |
| Chemical compound, drug | EdU | Thermo Fisher Scientific | #A10044 | |
| Chemical compound, drug | Cardiotoxin | Latoxan | Cat# L8102 | 10 µM |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | Cat# T5648-1G | |
| Others | DAPI | Vector Laboratories | Cat# H-1200 | |
| Others | MOM blocking | Vector Laboratories | Cat# BMK-2202 | |
| Sequence-based reagent | 5’-TGTGGATCACCTGAAAATGC | Eurofins | N/A | qPCR Hey1 (Forward primer) |
| Sequence-based reagent | 5’-ACCCCAAACTCCGATAGTCC | Eurofins | N/A | qPCR Hey1 (Reverse primer) |
| Sequence-based reagent | 5’-CAGCCCTTCGCAGATGCAA | Eurofins | N/A | qPCR Heyl (Forward primer) |
| Sequence-based reagent | 5’-CCAATCGTCGCAATTCAGAAAG | Eurofins | N/A | qPCR Heyl (Reverse primer) |
| Sequence-based reagent | 5’-GCTACTCCTGTTCCTGCTGC | Eurofins | N/A | qPCR Col5a1 (Forward primer) |
| Sequence-based reagent | 5’-TGAGGGCAAATTGTGAAAATC | Eurofins | N/A | qPCR Col5a1 (Reverse primer) |
| Sequence-based reagent | 5’-CCGGAGACTGGATCAGCTT | Eurofins | N/A | qPCR Col5a3 (Forward primer) |
| Sequence-based reagent | 5’-GCTTCCAGTACGTCCACAGG | Eurofins | N/A | qPCR Col5a3 (Reverse primer) |
| Sequence-based reagent | 5’-TACTTCGGGAAAGGCACCTA | Eurofins | N/A | qPCR Col6a1 (Forward primer) |
| Sequence-based reagent | 5’-TCGGTCACCACGATCAAGT | Eurofins | N/A | qPCR Col6a1 (Reverse primer) |
| Chemical compound, drug | Trizol LS reagent | Thermo Fisher Scientific | Cat# 10296028 | |
| Chemical compound, drug | RNeasy mini kit | QIAGEN | Cat# 74104 | |
| Software, algorithm | Photophop CC | https://www.adobe.com/products/photoshop.html | RRID:SCR_014199 | |
| Software, algorithm | Hybrid Cell count | https://www.keyence.com/ss/products/microscope/bz-x/products-info/quantify.jsp | ||
| Software, algorithm | UMI-tools | https://github.com/CGATOxford/UMI-tools | v.1.1.0 | |
| Software, algorithm | HISAT2 | https://ccb.jhu.edu/software/hisat2/index.shtml | v.2.1.0 | |
| Software, algorithm | featureCounts | http://subread.sourceforge.net/ | v.1.6.4 | |
| Software, algorithm | DESeq2 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | DOI: 10.18129/B9.bioc.DESeq2 | v.1.22.2 |