Fig 3. High mutant JAK2^V617F allele burden is detected in NSG mice transplanted with PMF-derived CD14⁺ cells.

The levels of human JAK2^V617F allele burden were measured in blood samples of NSG mice transplanted with CD14⁺/CD34^- BM cells obtained from PMF patient with a JAK2^V617F mutation. Depicted are the JAK2^V617F allele burdens of 15 mice transplanted with CD14⁺/CD34^- cells from 3 different PMF patients. The animals’ blood was obtained 2 weeks after transplantation and before the animals were euthanized (final bleed). Although there is apparent decline in the JAK2^V617F allele burden at the time of final bleed, the human mutant JAK2 variant was still detected at a significant measurable level several months after transplantation.