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. Author manuscript; available in PMC: 2020 Jan 4.
Published in final edited form as: Curr Cancer Drug Targets. 2019;19(10):838–851. doi: 10.2174/1568009619666190325144636

Figure 2. Clonogenic cell death caused by PCAIs.

Figure 2.

Lung cancer cells in culture were treated with PCAIs (1 – 5 μM) for 48 h, washed, trypsinized, counted and cultured in the absence of PCAIs for a further 12–14 days at 37°C. Cells that survived formed colonies which were fixed and stained with crystal violet. Representative images of colonies were captured (A) and then clonogenic survival was determined (B). The results are expressed as the means (± SEM, N = 4) relative to the controls. Significance (***p < 0.001) was determined by Student’s t-test.