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. Author manuscript; available in PMC: 2020 Jan 4.
Published in final edited form as: Curr Cancer Drug Targets. 2019;19(10):838–851. doi: 10.2174/1568009619666190325144636

Figure 8. PCAIs stimulate MAPK phosphorylation in A549 cells.

Figure 8.

Cells (2×106 in 60.8 cm2 wells) were seeded overnight and treated with the indicated concentrations of NSL-BA-040 and NSL-BA-055 for 48 h. They were rinsed with PBS, lysed with RIPA buffer mixed with 0.1%v/v cocktail protease inhibitors and SDS-PAGE sample buffer immediately added and boiled. The samples were then analyzed by western blot for P44/42 MAPK and β-Actin levels. Note the depletion of proteins in the samples treated with the more potent NSL-BA-055 likely due to apoptosis.