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. Author manuscript; available in PMC: 2019 Nov 8.
Published in final edited form as: Nature. 2019 May 8;569(7756):418–422. doi: 10.1038/s41586-019-1191-6

Extended Data Figure 6. Multinucleation and CM hypertrophy in miR-199a-treated pig hearts.

Extended Data Figure 6

a, Representative images of longitudinal sections stained with wheat germ agglutinin (WGA) to assess the number of nuclei per CM in the infarct border zone of AAV6-Control- and AAV6-miR-199a-treated animals (n=4 and n=6, respectively; analysis is from at least 7 high-resolution images acquired from at least 8 different regions of each heart), 12 days post MI. The right panels show the estimated number of nuclei for each cardiomyocyte. Scale bar: 50 µm. b, Additional representative images of mono- or bi-nucleated BrdU-positive CMs in the infarct border zone of AAV6-Control- and AAV6-miR-199a-treated animals, 12 days post MI. Scale bar: 50 µm. c, Cross-sectional area measurements of BrdU+ and BrdU- cardiomyocytes in AAV6-Control- and AAV6-miR-199a-treated pigs 12 days after surgery. Data are mean±SEM from the analysis of 4 pigs. d, Representative images of BrdU+ and BrdU- CM. Scale bar: 50 µm. The right panels are high magnification images of the indicated portions of the left images.