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. Author manuscript; available in PMC: 2019 Nov 8.
Published in final edited form as: Nature. 2019 May 8;569(7756):418–422. doi: 10.1038/s41586-019-1191-6

Figure 2. miR-199a delivery improves global and regional cardiac function.

Figure 2

a-d, LV ejection fraction (EF, %), stroke volume (ml), LV end-systolic volume (ml) and LV end-diastolic volume (ml) measured by cMRI in non-infarcted controls and infarcted animals at days 2 and 28 post-MI and either AAV6-Control or AAV6-miR-199a injection. Data are mean±SEM; the number of animals per group and time point is indicated. ns: not significant; *P<0.05 vs. AAV6-Control at the same time point; #P<0.05 vs. sham; $P<0.05 vs. day 2; two-way ANOVA with Bonferroni post-hoc. e, Example of cardiac short axis image with the tagging grid in diastole and systole. f, Subdivision of the LV short axis in 8 circumferential segments (left) and their correspondence with the infarct core, border zone and the remote zone (right). The syringe indicates the infarct border injected with AAVs. IS, inferoseptal; S, septal, AS, anteroseptal; A, anterior; AL, anterolateral; L, lateral; IL, inferolateral; I, inferior. g, h, Eight-segment curves corresponding to LV radial (LVErr) (g) and circumferential (LVEcc) (h) strain at 28 days after MI. Data are mean±SEM. *P<0.05 vs. AAV6-Control; #P<0.05 vs. sham; two-way ANOVA with Bonferroni post-hoc. The number of animals for the analysis is indicated in panels j and k. i, Schematic example of calculation of the area under curve (AUC) in arbitrary units. j, k, AUC for Err (j) and Ecc (k). Data are mean±SEM; the number of animals per group is indicated. *P<0.05 vs. AAV6-Control; #P<0.05 vs. sham; one-way ANOVA with Bonferroni post-hoc. l, Eight-segment curves corresponding to LV end-systolic wall thickening (LVWT) at 28 days after MI. Data are mean±SEM. *P<0.05 vs. AAV6-Control; #P<0.05 vs. sham; two-way ANOVA with Bonferroni post-hoc. The number of analysed animals is shown in panel m. m, AUC for LVWT. Data are mean±SEM; the number of animals per group is indicated. *P<0.05 vs. AAV6-Control; #P<0.05 vs. sham; one-way ANOVA with Bonferroni post-hoc.