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. Author manuscript; available in PMC: 2019 Nov 8.
Published in final edited form as: Nature. 2019 May 8;569(7756):418–422. doi: 10.1038/s41586-019-1191-6

Figure 4. Long-term expression of miR-199a induces progressive cardiac regeneration but causes sudden death.

Figure 4

a, LGE-cMRI representative images, from apex to base, of one AAV6-Control and one AAV6-miR-199a-treated pig heart at 1, 4 and 8 weeks after MI. The infarct area is counterstained in red; the corresponding original images without counterstaining are shown in Extended Data Fig. 9a. Similar cardiac repair results were observed in three pigs treated with miR-199a that survived 2 months after treatment. b, Kaplan Meier curve (log-rank test) showing mortality after MI and vector administration. The number of animals per group is indicated. c, Hematoxylin-eosin staining or immunostaining for the indicated antigens of the same cell cluster in consecutive tissue sections from an infarcted heart injected with AAV6-miR-199a at 8 weeks after treatment. Scale bar: 100 µm. d, In situ hybridisation of miR-199a-3p, scrambled and U6 LNA probes in pig heart sections with infiltrating cell cluster. Scale bar: 100 µm.