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Effect of phenylalanine intake on production of 13CO2 from phenylalanine oxidation (F13CO2) by DAAO in adults aged >65 y; n = 12 subjects, 66 DAAO studies. Biphase linear regression analysis of the F13CO2 data identified a breakpoint of 9.03 mg .kg−1 .d−1, which represents the estimated mean phenylalanine requirement (R2 = 0.79). The safe population intake estimated by determining the upper 95% CIs of the breakpoint was 15.9 mg .kg−1 .d−1. DAAO, direct amino acid oxidation.
