Fig. 2. Developing CGNs express NgR1, NgR2 and p75^NTR but not MAG.

a–b Micrographs of representative P7 CGNs double-labelled with anti-MAG (a), anti-NgR1 or anti-p75^NTR (b) combined with anti- β III tubulin and counterstained with DAPI after 24 h in culture. Scale bars, 20 μm. c–e NgR1 mRNA (c), NgR2 mRNA (d) and p75^NTR mRNA (e) levels relative to the reference mRNA of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in P7 CGNs cultured for 1, 2 and 3 days in vitro. Mean ± s.e.m of data from eight separate cultures is shown