Fig. 6. Secretase inhibitors rescue the effect of DGBP on apoptosis.

a, b Annexin V staining of Molt-4 or Jurkat cells treated with DGBP and co-treated with or without (a) DAPT or (b) MK-0752 for 72 h. Graphs represent means and standard deviations of three independent experiments. Statistical significance was determined by two-way ANOVA with Tukey’s post hoc analysis. *p < 0.05 versus untreated controls. ^‡p < 0.05 versus DGBP-treated condition. c, d Western blot of full-length Notch1 and NICD in Molt-4 cells following 72 h of treatment with DGBP and co-treatment with or without either the GGDPS product GGPP (c) or the caspase inhibitor Z-VAD-FMK (d). Tubulin is shown as a loading control. e, f Western blots of Notch1 and NICD after 72 h of treatment with DGBP and co-treatment with or without DAPT in Molt-4 (e) and Jurkat (f) cells. All Western blots are representative of three independent experiments