Fig. 1. The downregulation of MiR-532-3p in colorectal cancer.

a The expression patterns of eight miRNAs in normal colorectal mucosa (N), colorectal adenoma (CRA), and colorectal adenocarcinoma (CRC) tissues with a public expression profiling dataset GSE41655. b The normalized miRNAs expression levels of miR-532-3p and miR-532-5p in GSE41655 dataset. c Expression of miR-532-3p was verified in 54 pairs of CRC tissues from a clinical cohort, which was normalized against an endogenous U6 RNA control. d Relative miR-532-3p expression in CRC compared with that in the paired normal colorectal mucosa, the fold change was normalized with log₂(CRC/N). e Expression of miR-532-3p in seven colorectal cancer cell lines and a colorectal mucosal cell line FHC. f Detection of miR-532-3p in HT29 cells transfected with miR-532-3p mimics and RKO cells with miR-532-3p inhibitors by qPCR. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant