Fig. 3. MiR-532-3p suppresses cell proliferation and migration of CRC.

a MiR-532-3p mimics decreased the cell growth and the inhibitors restored the proliferation in HT29 and RKO cells by CCK-8. b Clone formation assay showed a decrease or increase in the proliferation of HT29 and RKO cells after transfection with miR-532-3p mimics and inhibitors as indicated. c Subcutaneous tumor growth curve of HT29 cells and RKO cells infected with NC, miR-532-3p, i-NC, and i-miR-532-3p lentivirus as indicated. d The transwell assay for HT29-NC, HT29-miR-532-3p, RKO-i-NC, RKO-i-miR-532-3p groups. e The wound-healing assay in HT29 and RKO cells transfected with miR-532-3p mimics or inhibitors as indicated. f Western blot analysis of EMT markers and Smad, MAPK proteins in HT29 and RKO cells. *p < 0.05, **p < 0.01, ***p < 0.001