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. 2019 Sep 30;10(10):739. doi: 10.1038/s41419-019-1962-x

Fig. 5. ETS1 activates Wnt/β-catenin pathway by directly regulating TGM2 transcription and induces CRC progression.

Fig. 5

a Western blot analysis of ETS1, TGM2, β-catenin in RKO cells transfected with TGM2, ETS1 plasmids, and P-Enter used as control. b Spearman’s correlation analysis showing a positive correlation between ETS1 and TGM2 mRNA levels according to TCGA datasets. c Chip-qPCR analysis of ETS1-binding regions in TGM2 promoter showing the important sites including −186/−13 and −358/−252. d Western blot analysis of β-catenin and downstream molecules of Wnt/β-catenin signaling in HT29-miR-532-3p cells and RKO-i-miR-532-3p cells. e Apoptosis analysis of RKO-P-Enter, RKO-ETS1, RKO-TGM2 cells after treatment with 5-Fu, cisplatin. f The CCK-8 assay was performed to detect the effect of ETS1 and TGM2 in the proliferation ability of RKO cells. g Wound-healing assay evaluating the motility of RKO-P-Enter, RKO-ETS1, RKO-TGM2 cells. *p < 0.05, **p < 0.01, n.s. non-significant