. 2019 Jul 31;32(5):745–755. doi: 10.1007/s10534-019-00207-2

Table 1.

Michaelis–Menten analyses for hinokitiol and Fe

Concentration constant	Concentration varied	Vmax	Km
500 nM hinokitiol	Fe²⁺	2.2 ± 0.1	448 ± 66
1000 nM hinokitiol	Fe²⁺	4.3 ± 0.2	455 ± 86
500 nM hinokitiol	Fe³⁺	24.1 ± 3.3	1790 ± 500
1000 nM hinokitiol	Fe³⁺	87 ± 8	3010 ± 510
200 nM Fe²⁺	Hinokitiol	5.8 ± 1.2	6000 ± 2400
500 nM Fe²⁺	Hinokitiol	15.7 ± 5.4	13,200 ± 7000
200 nM Fe³⁺	Hinokitiol	6.2 ± 1.1	1620 ± 848
500 nM Fe³⁺	Hinokitiol	10.1 ± 0.6	545 ± 117

Data for 1000 nM hinokitiol resulted from fitting the data in Fig. 4 as described in its legend. Remaining rows came from similar analyses (not shown) with the concentration of hinokitiol unvaried in each of the top four rows while that for iron was unvaried in each of the last four rows. Vmax units are nEq/L min; km units are nEq/L