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. 2019 May 21;56(11):1177–1189. doi: 10.1007/s00592-019-01366-x

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Effect of DATS on inhibition of high glucose-induced HUVEC apoptosis in vitro. a TUNEL assay. HUVECs were seeded, treated with different agents, then grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to the TUNEL assay. The images show DAPI-stained (blue) and TUNEL-positive cells (pink); scale bar = 25 μm. b Graph shows the quantified HUVEC apoptosis data. c Caspase-3 activity assay. HUVECs were seeded, treated with different agents, then grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to the caspase-3 activity assay. d Graph shows the quantified HUVEC apoptosis data determined by flow cytometry. e Apoptosis was determined by flow cytometry. HUVECs were seeded, treated with different agents, grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to annexin V/PI staining for flow cytometry detection. The data are expressed as mean ± SEM (n = 3). *p < 0.05 versus the NG group and ^#p < 0.05 versus the HG group