Fig. 3.

Effect of DATS on inhibition of the high glucose-induced HUVEC mitochondrial apoptotic pathway. a JC-1 staining of the mitochondrial membrane potential, a hallmark of mitochondrial apoptosis. HUVECs were seeded onto a coverslip, treated with different agents, then grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to the assay. Scale bar = 25 μm. b Quantified data described in (a). c–f Western blot. HUVECs were seeded, treated with different agents, then grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to western blot analysis of cytochrome c (Cytc), Bcl-2, and Bax, markers of mitochondria-related cell apoptosis. g–i Western blot. HUVECs were seeded, treated with different agents, then grown under normal glucose (NG) or high-glucose (HG) conditions, and subjected to western blot analysis of cytochrome c in the mitochondrial and cytoplasmic fractions. The data are represented as mean ± SEM (n = 3). *p < 0.05 versus the NG group and ^#p < 0.05 versus the HG group