Fig. 5.

Effect of DATS on protection of high glucose-induced Drp1 and HUVEC mitochondrial fission via AMPK activation. a–d, m–p Western blot. HUVECs were seeded, treated with different agents, and subjected to western blot analysis of p-AMPK, AMPK, p-ACC, ACC, and Drp1 proteins. e, g, q, t Intracellular and mitochondrial ROS assay. f, h, r, s Quantified data described in (e, g, q), and t. i, u JC-1 staining of the mitochondrial membrane potential. j, v Quantified data described in (i) and (u). k, x TUNEL assay and flow cytometry. l, w Quantified data described in (k) and (x). Representative photographs of DAPI (blue) and TUNEL (pink) staining; scale bar = 25 μm. The data are represented as mean ± SEM (n = 3). *p < 0.05 versus the NG group, ^#p < 0.05 versus the HG group, and ^&p < 0.05 versus the HG plus DATS group