Fig. 4.

SDS-PAGE showing the protein crude extracts from REG19, JA-Bs34 to JA-Bs40 and from REG19 carrying the plasmid pHWG1132. Crude extracts were prepared from cells induced by glucitol for 16 h, cleared by centrifugation and the amount of 15 μg cleared crude extract proteins were applied on the SDS-gel in each line. The GanA protein of 79.8 kDa is indicated by the arrow