TABLE 3.

Fold changes of CTX-induced (1/2 MIC) increased conjugation transfer frequency, relative to untreated strains, using MG1655/pTF2 (WT) and the mutants as donors and J53-2 as recipient.

Donor	Conjugation time (min)	Conjugation transfer frequency (Control)a	Conjugation transfer frequency (CTX)a	Plasmid transfer fold differenceb
WT	30	3.90 × 10–5 ± 1.05 × 10–5	9.75 × 10–4 ± 1.46 × 10–4	25.00∗∗
	60	3.00 × 10–4 ± 0.49 × 10–4	1.23 × 10–2 ± 0.34 × 10–2	41.00∗
ΔrfaH	30	1.13 × 10–5 ± 0.32 × 10–5	0.87 × 10–5 ± 0.26 × 10–5	0.77
	60	1.14 × 10–4 ± 0.12 × 10–4	1.06 × 10–4 ± 0.39 × 10–4	0.93
ΔyhiN	30	2.48 × 10–5 ± 0.96 × 10–5	3.64 × 10–4 ± 0.39 × 10–4	14.68∗∗
	60	5.25 × 10–4 ± 1.24 × 10–4	2.86 × 10–3 ± 0.60 × 10–3	4.50∗
ΔwaaP	30	2.33 × 10–5 ± 0.65 × 10–5	3.46 × 10–4 ± 0.72 × 10–4	14.85∗
	60	3.29 × 10–4 ± 0.79 × 10–4	3.09 × 10–3 ± 0.97 × 10–3	9.57
ΔwaaQ	30	2.97 × 10–5 ± 1.04 × 10–5	3.23 × 10–5 ± 0.67 × 10–5	1.09
	60	2.73 × 10–4 ± 0.60 × 10–4	4.47 × 10–3 ± 1.44 × 10–3	16.37
Δgnd	30	2.44 × 10–5 ± 0.64 × 10–5	3.16 × 10–4 ± 0.65 × 10–4	12.95∗
	60	3.59 × 10–4 ± 0.88 × 10–4	5.47 × 10–3 ± 0.4707 × 10–3	15.24∗∗
Δpgl	30	1.16 × 10–5 ± 0.24 × 10–5	1.26 × 10–4 ± 0.24 × 10–4	10.86∗
	60	2.27 × 10–4 ± 0.48 × 10–4	2.31 × 10–3 ± 0.41 × 10–3	10.18∗

^aThe results shown are from two biological replicates with three technical replicates each, the data shown represent the mean ± standard deviation. ^bThe stars indicate statistical significance at level: ^∗P ≤ 0.05, ^∗∗P ≤ 0.01.