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. 2019 Sep 24;10:1227. doi: 10.3389/fphys.2019.01227

FIGURE 5.

FIGURE 5

Inhibition of ovalbumin (OVA)-induced oxidative stress by Aquatic exercise (AE). (A) Contents of malondialdehyde (MDA), (B) glutathione (GSH), and (C) superoxide dismutase (SOD) activity in the lung tissues. (D) Reduction in levels of phosphorylated p-ERK, p-p38, and p-JNK by AE. The total protein isolated from lung tissues was subjected to Western blot analysis using anti-p-ERK, anti-ERK, anti-p-p38, anti-p38, anti-p-JNK, and anti-JNK antibodies. (E) Representative bands of NF-E2-related factor 2 (Nrf2) in the nuclear and cytosol, as examined by western blot analysis in lung tissues. The bar graphs depict a relative density of immuno-positive bands. The anti-Lamin B1 or anti-β-actin antibodies were used as an internal control. (F) Representative protein bands of heme oxygenase-1 (HO-1), as determined by western blot analysis in lung tissues. The bar graphs depict a relative density of immuno-positive bands. The anti-β-actin antibody was used as an internal control. The bar graphs depict a relative density of immuno-positive bands. Data are represented as the means ± SEM. †p < 0.05, compared to control. ‡p < 0.05, compared to OVA. § p < 0.05, compared to OVA + Walking.