FIGURE 6.

Reduction of ovalbumin (OVA)-induced airway inflammation by Aquatic exercise (AE). (A) Levels of tumor necrosis factor (TNF)-α, IL-6, and prostaglandin E₂ (PGE₂) in bronchoalveolar lavage fluid (BALF). (B) Inhibition of OVA-induced NF-κB nuclear translocation by AE. Nuclear and cytoplasmic fractions were extracted and subjected to Western blot analysis using the anti-NF-κB p65 antibody. The anti-Lamin B1 or anti-β-actin antibodies were used as an internal control. The bar graphs depict a relative density of immuno-positive bands. Data are represented as the means ± SEM. ^†p < 0.05, compared to control. ^‡p < 0.05, compared to OVA. ^§ p < 0.05, compared to OVA + Walking.