Figure 2.

Incidence of fragmented and denervated NMJs are unchanged in adult and aged VKD^Het mice. NMJs were visualized in EDL muscles from 1, 5, and 17 month-old control (A) and VKD^Het (B) mice using YFP (green) and α-bungarotoxin (red). The percentage of fragmented NMJs is similar between control and VKD^Het mice at each age examined (C). The percentage of fully denervated NMJs is similar between control and VKD^Het mice at each age examined (D). Represented as mean ± SEM. Scale bar = 20 μm. Representative images are from 5 month-old mice. Only male mice were used for these experiments. Control n = 3; VKD^Het n = 3 for each age group. At least 50 NMJs were analyzed per animal. Black circles represent individual data points.