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Absorption spectra and cofactors of VvHmpA. (A) The absorption spectra of the purified VvHmpA in the reduced (solid line), NO-bound (dashed line), and oxidized (spotted line) states were measured using a UV–vis spectrophotometer. The numbers above the peaks indicate the wavelengths of the observed Soret peaks. (B) The ratios of heme and FAD per one molecule of purified and reconstituted VvHmpA.
