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. 2019 Sep 30;9:14001. doi: 10.1038/s41598-019-50440-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volcano plot (a) and heatmap (b) for comparisons between the L and S strains. (a) Volcano plot showing log2 FC (x-axis) and log10 FDR corrected P-value (y-axis) points of all the expressed genes in the Tcas5.2 reference genome using Microsoft Office Excel. The orange dots represent 232 DEGs of the L strain (FC ≥ 1.5 and q < 0.05), whereas the green dots represent 286 DEGs of the S strain (FC ≤ 0.67 and q < 0.05). (b) A heatmap showing 68 DEGs with defined locus names was created using the R package “gplots”. The color gradient represents high-expression (red) to low-expression (blue) by calculating log2 (FPKM + 1).