Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 30;9:13991. doi: 10.1038/s41598-019-50614-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

T_FH1-like cells express both IFN-γ and IL-21. (A) qRT-PCR analysis of Ifng and Il21 expression in the indicated cell populations following stimulation with PMA/Ionomycin for 2.5 hrs. The data were normalized to Rps18 and presented as fold change relative to the T_FH1-like sample (mean of n = 5 ± s.e.m.). (B) Flow cytometry analysis of intracellular expression of IL-21 and IFN-γ in the indicated cell populations. Shown are representative data from four independent experiments. (C) Percentage of IFN-γ⁺IL-21⁺ cells as assessed by flow cytometry analysis (mean of n = 4 ± s.e.m.). **P < 0.01, ***P < 0.001; one-way ANOVA with Tukey multiple-comparison test.