Fig. 2. The TRIM71-NHL domain interacts with the TA domain of mutant p53-R273H.

a Mapping the TRIM71 binding domain of mtp53-R273H. HCT116^p53−/− cells were transfected with the plasmid encoding Flag-p53-R273H fragment, aa 1–300, aa 101–300 or aa 301–393, along with the Myc-TRIM71-encoded plasmid. Co-IP assays were performed using the anti-Flag antibody followed by IB with the anti-Myc antibody. b HCT116^p53−/− cells were transfected with plasmids encoding Myc-TRIM71 and Flag-p53-(aa 1–100) followed by co-IP-IB assays using antibodies as indicated. c Mapping the mtp53-R273H binding domain of TRIM71. HCT116^p53−/− cells were transfected with the plasmids encoding each Myc-TRIM71 fragment along with the Flag-p53-R273H-expressing plasmid. Co-IP-IB assays were performed using antibodies as indicated. d A schematic diagram of TRIM71-binding region on mtp53-R273H. TAD, Transactivation domain (1–42); DBD, DNA-binding domain (101–306); OD, Oligomerization domain (307–355); CTD: C-terminal regulatory domain. e A schematic diagram of mtp53-R273H binding region on TRIM71