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. 2019 Sep 30;10(10):737. doi: 10.1038/s41419-019-1977-3

Fig. 5. TRIM71 suppresses proliferation and invasion of ovarian cancer cells through inhibition of mutant p53s.

Fig. 5

a, b Lentivirus-based overexpression of TRIM71 inhibits proliferation of OVCA420 and ES-2 cells by the CCK-8 assays. c, d Lentivirus-based overexpression of TRIM71 inhibits invasion of OVCA420 and ES-2 cells by the transwell assays. The representative images and quantification analysis are shown in c, d, respectively. e, f Knockdown of mtp53 abrogates TRIM71 depletion-induced ovarian cancer cell proliferation. OVCA420 cells e and ES-2 cells stably expressing the ctrl-cas9 or TRIM71-cas9 f were transfected with indicated siRNAs followed by the CCK-8 assays. g–j Knockdown of mtp53 abrogates TRIM71 depletion-induced ovarian cancer cell invasion. OVCA420 cells g, h and ES-2 cells stably expressing the ctrl-cas9 or TRIM71-cas9 i, j were transfected with indicated siRNAs followed by transwell assays. The representative images and quantification analysis are shown in g, i and h, j, respectively. k, l Representative IB assays show the knockdown efficiency of TRIM71 and/or mtp53 in OVCA420 cells k and ES-2 cells l