Fig. 4. IDH1-AS1 regulated ATG5-mediated autophagy in PCa.

a Top 200 mRNAs that were significantly downregulated in IDH1-AS1-silenced cells were screened out by microarray analysis. b GO analysis of IDH1-AS1-reulated 200 mRNAs. c ATG5 expression level in cells transfected with IDH1-AS1-specific shRNAs and control shRNA was measured using qRT-PCR examination. The level of autophagy-related proteins, LC3 fluorescence intensity and the ratio of autophagosome were detected successively by western blotting (d), LC3 immunofluorescence (e), and MDC staining (f). g ATG5 expression in PCa tissues and its expression association with IDH1-AS1 expression. *P < 0.05, **P < 0.01. Scale bars for LC3 immunofluorescence and MDC staining equal 200 μm