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. 2019 Sep 30;10(10):734. doi: 10.1038/s41419-019-1932-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Pull-down silver staining showing proteins which could interact with IDH1-AS1. b The interaction between PTBP3 and IDH1-AS1 or ATG5 was proved by RIP assay. c, d Overexpression of PTBP3 in LNCaP and VCaP cells and relative ATG5 expression were detected by qRT-PCR examination. e ATG5 mRNA stability was assessed in PCa cells treated with actinomycin D after overexpression of PTBP3. f RIP assay was applied to detect the interaction between ATG5 and PTBP3 in PCa cells after silencing of IDH1-AS1. g ATG5 mRNA stability was evaluated in cells transfected with shIDH1-AS1 or co-transfected with shIDH1-AS1 + PTBP3. *P < 0.05, **P < 0.01