Fig. 1.

Minimal adhesive unit of PCDH1 is EC1–4. a–g Protein G beads coated with full length (a) and truncated versions (b–g) of the PCDH1 extracellular domain imaged after incubation for 1 h followed by rocking for 2 min in the presence of calcium. Scale bar: 500 µm. h Western blot shows efficient expression of full length and truncated PCDH1 EC repeats. i Mean aggregate size for full length and truncated fragments of PCDH1 at T0 (t = 0 min), after 1 h of incubation, T60 (t = 60 min) followed by rocking for 1 min (R1) and 2 min (R2). Error bars are standard error of the mean (n = 4 independent experiments for all aggregation assays and constructs except for PCDH1 EC1-7Fc with n = 3 independent experiments)