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. 2019 Sep 24;6:83. doi: 10.3389/fmolb.2019.00083

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Copyright © 2019 Salnikov, Aisenbrey, Pokrandt, Brügger and Bechinger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proton-decoupled cross-polarization ¹⁵N solid-state NMR spectra of [¹⁵N-Leu15]-DQB1 reconstituted into 40 mg of phospholipids oriented on glass plates. The membranes are made of POPC (A,B), POPC/SM-C18 95/5 mole/mole (C,D), or DMPC (E,F). The peptide-to-lipid ratio is 1 mole% in the top row (A,C,E) and 2 mole% in the second row (B,D,F). Reconstitution followed the protocol described in Kemayo Koumkoua et al. (2014). The membrane normal is aligned parallel to the magnetic field direction. The measurements were performed at room temperature (A–D) or at 310 K for the DMPC membranes (E,F).